Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Survivin 2α expression (relative to normal tissue).

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing

Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Expression of survivin splice variants in medulloblastoma (relative to survivin)

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing

Journal: bioRxiv

Article Title: Direct measurement of RNA Polymerase II hypertranscription in cancer FFPE samples

doi: 10.1101/2024.02.28.582647

Figure Lengend Snippet: Pathology classification, age and sex were provided by the vendor (BioChain). Each image spans the width of a standard charged microscope slide, where the tissue is visible under the paraffin skin. On-slide RNAPII-Ser5p FFPE-CUTAC was applied to slides in parallel, using a total of four slides each for 100 separate samples in all to produce the data analyzed in this study.

Article Snippet: The following pairs of human tumor and adjacent normal 5 μm tissue sections from single FFPE blocks were purchased from Biochain, Inc: Breast Normal/Tumor cat. no. T8235086PP/PT; Colon Normal/Tumor cat. no. T8235090PP/PT; Kidney Normal/Tumor cat. no. T8235142PP/PT; Liver Normal/Tumor cat. no. T8235149PP/PT; Lung Normal/Tumor cat. no. T8235152PP/PT; Rectum Normal/Tumor cat. no. T8235206PP/PT; Stomach Normal/Tumor cat. no. T8235248PP/PT.

Techniques: Microscopy

Journal: Biomedicines

Article Title: Potent Small-Molecule Inhibitors Targeting Acetylated Microtubules as Anticancer Agents Against Triple-Negative Breast Cancer

doi: 10.3390/biomedicines8090338

Figure Lengend Snippet: Inhibition of triple-negative breast cancer (TNBC) progression by blocking microtubule acetylation. ( A ) Representative images showing acetyl-α-tubulin expression in various subtypes of human breast cancer. Scale bar, 500 µm. The intensity was analyzed using Aperio Image Scope software. ( B ) Expression of microtubule acetylation in breast cancer cell lines (upper panel) categorized according to the subtype (lower panel). ( C ) Confirmation of acetyl-α-tubulin expression in alpha-tubulin acetyltransferase 1 (αTAT1) Knockout (KO) MDA-MB-231 cell lysates. ( D ) Anchorage-independent growth assays using αTAT1 KO MDA-MB-231 cells for 3 weeks. The number of colonies was quantified using ImageJ software. Scale bar, 1 mm. ***, p ≤ 0.001. ( E ) Mock and αTAT1 KO MDA-MB-231 cells were injected into the mammary fat pad of non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice ( n = 5, each). *, p ≤ 0.05; ***, p ≤ 0.001.

Article Snippet: Paraffin-embedded human breast cancer tissue samples and patient pathological data were obtained from US Biomax, Inc., Derwood, MD, USA (BR10011).

Techniques: Inhibition, Blocking Assay, Expressing, Software, Knock-Out, Injection